Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates

1980405 citationsJournal Articlebronze Open Access

Authors

A. John Barrett · Tissue Genetics (United States)

Abstract

Benzyloxycarbonyl-phenylalanyl-arginine 4-methyl-7-coumarylamide was found to be an excellent substrate for the fluorimetric assay of cathepsin B, and arginine 4-methyl-7-coumarylamide for cathepsin H. Procedures were developed that are very convenient, and avoid the hazards associated with the use of naphthylamides.

Topics & Keywords

Protease and Inhibitor Mechanisms S100 Proteins and Annexins Venomous Animal Envenomation and Studies

Publication Details

Published in: Biochemical Journal

Volume 187, Issue 3, pp. 909-912

DOI: 10.1042/bj1870909

Field-Weighted Citation Impact: 6.32

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Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates

Authors

Abstract

Topics & Keywords

Publication Details