MethPrimer: designing primers for methylation PCRs

20022,810 citationsJournal Articlebronze Open Access

Authors

Long-Cheng Li · San Francisco VA Medical Center

Rajvir Dahiya · San Francisco VA Medical Center

Abstract

MethPrimer, based on Primer 3, is a program for designing PCR primers for methylation mapping. It first takes a DNA sequence as its input and searches the sequence for potential CpG islands. Primers are then picked around the predicted CpG islands or around regions specified by users. MethPrimer can design primers for BSP and MSP. Results of primer selection are delivered through a web browser in text and in graphic view.

Topics & Keywords

Epigenetics and DNA Methylation Cancer-related gene regulation RNA modifications and cancer

UN Sustainable Development Goals

Life below water

Publication Details

Published in: Bioinformatics

Volume 18, Issue 11, pp. 1427-1431

DOI: 10.1093/bioinformatics/18.11.1427

Field-Weighted Citation Impact: 6.65