Femtosecond laser-assisted keratoplasty: full and partial-thickness cut wound strength and endothelial cell loss across a variety of wound patterns

Partial thickness zigzag, top hat and mushroom-style partial FLAK incisions left an intact tissue wall with high resistance to rupture, whereas full-thickness cuts were more variable. Laser trephination and eye bank handling protocol for donor FLAK buttons leads to moderate peripheral endothelial cell loss in tissue with both complete and partial cuts.