Biosynthesis of the Cyclotide Kalata B1 by Using Protein Splicing

2006121 citationsJournal Article

Authors

Ahn‐Tuyet Tran · Lawrence Livermore National Laboratory

Julio A. Camarero · Lawrence Livermore National Laboratory

Abstract

The tides are turning: Kalata B1 (KB1) is the prototypical member of an exceptionally stable and resistant family of plant-derived miniproteins called cyclotides. The biosynthesis of KB1 and a small library of KB1-based cyclotides (variation occurs in five solvent-exposed loops) in E. coli has been performed by using recombinant-DNA expression techniques and protein splicing.

Topics & Keywords

Biochemical and Structural Characterization Glycosylation and Glycoproteins Research Phytoplasmas and Hemiptera pathogens

UN Sustainable Development Goals

Life below water

Publication Details

Published in: Angewandte Chemie International Edition

Volume 45, Issue 6, pp. 973-976

DOI: 10.1002/anie.200503882

Field-Weighted Citation Impact: 4.24