Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae

20101,647 citationsJournal Article

Authors

Caroline Dallenne · Sorbonne Université

Anaelle Da Costa · Sorbonne Université

Dominique Decré · Sorbonne Université

Christine F. Favier · Bio-Rad (France)

Guillaume Arlet · Hôpital Tenon

Abstract

We report here the development of a useful method composed of a set of six multiplex PCRs and one simplex PCR for the rapid screening of the most frequently encountered beta-lactamases. This method allowed direct sequencing from the PCR products.

Topics & Keywords

Antibiotic Resistance in Bacteria Enterobacteriaceae and Cronobacter Research Pharmaceutical and Antibiotic Environmental Impacts

Publication Details

Published in: Journal of Antimicrobial Chemotherapy

Volume 65, Issue 3, pp. 490-495

DOI: 10.1093/jac/dkp498

Field-Weighted Citation Impact: 17.96

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Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae

Authors

Abstract

Topics & Keywords

Publication Details