Sequencing templates – shotgun clone isolation versus amplification approaches

Abstract Preparation of good‐quality DNA is essential for obtaining high‐quality DNA sequence data. Alkaline lysis remains the most popular method for isolating small and large DNA constructs, but new techniques including solid‐phase immobilization and rolling circle amplification offer alternatives to the traditional approaches. Quality, quantity, and consistency of isolated DNA vary from method to method, and the choice of DNA isolation method should be carefully considered, particularly when a variety of vectors or host strains will be used.