Synthesis and Purification of Oligonucleotide N3′→P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras

This unit describes the synthesis and purification of oligonucleotide N3'-->P5' phosphoramidates, wherein each 3'-oxygen is replaced by a 3'-amine in the 2'-deoxyribose ring. The synthesis of required monomers and application of the method to preparation of phosphodiester- and phosphorothioate-containing chimera of phosphoramidate is also reported.