Spectroscopy of Single Hemoglobin Molecules by Surface Enhanced Raman Scattering

19992,330 citationsJournal Article

Authors

Hongxing Xu · Chalmers University of Technology

Erik J. Bjerneld · Chalmers University of Technology

Mikael Käll · Chalmers University of Technology

L. Börjesson · Chalmers University of Technology

Abstract

We demonstrate the detection of molecular vibrations in single hemoglobin (Hb) protein molecules attached to isolated and immobilized silver nanoparticles by surface enhanced Raman scattering (SERS). A comparison between calculation and experiment indicates that electromagnetic field effects dominate the surface enhancement, and that single molecule Hb SERS is possible only for molecules situated between Ag particles. The vibrational spectra exhibit temporal fluctuations of unknown origin which appear to be characteristic of the single molecule detection limit.

Topics & Keywords

Gold and Silver Nanoparticles Synthesis and Applications Protein Interaction Studies and Fluorescence Analysis Spectroscopy and Quantum Chemical Studies

Publication Details

Published in: Physical Review Letters

Volume 83, Issue 21, pp. 4357-4360

DOI: 10.1103/physrevlett.83.4357

Field-Weighted Citation Impact: 15.92

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Spectroscopy of Single Hemoglobin Molecules by Surface Enhanced Raman Scattering

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Abstract

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Publication Details