Atlas-Based Indexing of Brain Sections via 2-D to 3-D Image Registration

A 2-D to 3-D nonlinear intensity-based registration method is proposed in which the alignment of histological brain sections with a volumetric brain atlas is performed. First, sparsely cut brain sections were linearly matched with an oblique slice automatically extracted from the atlas. Second, a planar-to-curved surface alignment was employed in order to match each section with its corresponding image overlaid on a curved-surface within the atlas. For the latter, a PDE-based registration technique was developed that is driven by a local normalized-mutual-information similarity measure. We demonstrate the method and evaluate its performance with simulated and real data experiments. An atlas-guided segmentation of mouse brains' hippocampal complex, retrieved from the Mouse Brain Library (MBL) database, is demonstrated with the proposed algorithm.