Genomically Recoded Organisms Expand Biological Functions

2013861 citationsJournal Articlegreen Open Access

Authors

J. G. Lajoie · Harvard University

Alexis J. Rovner · Yale University

Daniel B. Goodman · Harvard University

Hans R. Aerni · Yale University

Adrian D. Haimovich · Yale University

Gleb Kuznetsov · Harvard University

Jaron A. M. Mercer ·

Abstract

We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.

Topics & Keywords

CRISPR and Genetic Engineering Animal Genetics and Reproduction Genetically Modified Organisms Research

Publication Details

Published in: Science

Volume 342, Issue 6156, pp. 357-360

DOI: 10.1126/science.1241459

Field-Weighted Citation Impact: 32.89

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Genomically Recoded Organisms Expand Biological Functions

Authors

Abstract

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Publication Details