Tracking down the sources of experimental contamination in microbiome studies

2014208 citationsJournal Articlegold Open Access

Authors

Sophie Weiss · University of Colorado Boulder

Amnon Amir · University of Colorado Boulder

Embriette R. Hyde · University of Colorado Boulder

Jessica L. Metcalf · University of Colorado Boulder

Se Jin Song · University of Colorado Boulder

Rob Knight · University of Colorado Boulder

Abstract

A recent report warns that DNA extraction kits and other laboratory reagents are considerable sources of contamination in microbiome experiments. The issue of contamination is particularly problematic for samples of low biomass.

Topics & Keywords

Gut microbiota and health Metabolomics and Mass Spectrometry Studies Microbial Community Ecology and Physiology

UN Sustainable Development Goals

Clean water and sanitation

Publication Details

Published in: Genome biology

Volume 15, Issue 12, pp. 564-564

DOI: 10.1186/s13059-014-0564-2

Field-Weighted Citation Impact: 5.12