Structure and Fuction of Succinoyl Trehalose Lipid on Differentiation-Induction of Human Promyelocytic Leukemia Cell Line HL-60

Four analogues of Succinoyl Trehalose Lipid-3 (STL-3) that have even- or odd-chain, unsaturated or halogenated fatty acids were compared to examine their abilities of inhibiting the growth and of inducing the differentiation of a human promyelocytic leukemia cell line HL-60. The optimal concentration of STL-3 for such activities was around the critical micelle concentration (CMC) of STL-3. The STL-3 analogues with even- or odd-chain or unsaturated fatty acids showed the potent growth inhibition and the inductions of differentiation of HL-60 cells, as evaluated by NBT reducing enzymatic activity and appearence of CD36 antigen. By contrast, the STL-3 analogue with halogenated fatty acids exhibited the weakest, but significant activities of inhibiting the cell growth and inducing of differentiation of HL-60 cells. Collectively, these results indicate that the induced activities of STL-3 for growth-inhibition and cell-differentiation depend on the structure of hydrophobic moiety.