In vitro Antibacterial Activity of Vitis vinifera Leaf Extracts against some Pathogenic Bacterial Strains

2 Abstract: Grape (Vitis vinifera) usually grows well in temperate countries. Many study show that consumption of grape and various other parts of the fruit, especially the seeds, provides many health benefits. The purpose of this study was to explore the antimicrobial activity of Vitis vinifera leaf extract. Sample consisting of fresh healthy leaves that were tested for their properties to inhibit growth of four species of bacteria, namely, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. Disc diffusion test method was used to determine the ability of the extract to inhibit the bacteria growth. The inhibition zones against the tested bacteria were ranging from 23.7 to 30 mm. The highest zone of inhibition produced by hot water extract S. aureus was (30 mm), E. faecalis (28.9 mm), E. coli was (28 mm) and P. aeruginosa (23.7mm). The extract of Vitis vinifera leaf was found to be valuable antibacterial agent. Leaves extract was able to inhibit the growth of S. aureus, E. faecalis, E. coli and P. aeruginosa, but their inhibitory activity against P. aeruginosa was less. This study confirms that Vitis vinifera leaf extract has antimicrobial activity that could be beneficial to human health.