Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions

200958 citationsJournal Article

Authors

Marie-Ève Gingras · Centre hospitalier universitaire de Québec

Abstract

Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA-protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.

Topics & Keywords

DNA and Nucleic Acid Chemistry RNA and protein synthesis mechanisms Advanced biosensing and bioanalysis techniques

Publication Details

Published in: Methods in molecular biology

Volume 543, pp. 15-35

DOI: 10.1007/978-1-60327-015-1_2

Field-Weighted Citation Impact: 3.62

Command Palette

Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions

Authors

Abstract

Topics & Keywords

Publication Details