Rapid Detection of Phenol Using a Membrane Containing Laccase Nanoflowers

Like a red, red rose: Rapid and sensitive detection of phenol in water was achieved using laccase nanoflowers to catalyze the oxidative coupling of phenol to 4-aminopyrine to form an antipyrine dye, which was detected by absorption at 495 nm. The analysis was performed using a commercial syringe filter, in which the membrane with a pore size of 0.2 μm accommodated the laccase nanoflowers of 4 μm in diameter.