Identification of Psychoactive Degradants of Cannabidiol in Simulated Gastric and Physiological Fluid

<b><i>Introduction:</i></b> In recent research, orally administered cannabidiol (CBD) showed a relatively high incidence of somnolence in a pediatric population. Previous work has suggested that when CBD is exposed to an acidic environment, it degrades to Δ⁹-tetrahydrocannabinol (THC) and other psychoactive cannabinoids. To gain a better understanding of quantitative exposure, we completed an <i>in vitro</i> study by evaluating the formation of psychoactive cannabinoids when CBD is exposed to simulated gastric fluid (SGF). <b><i>Methods:</i></b> Materials included synthetic CBD, Δ⁸-THC, and Δ⁹-THC. Linearity was demonstrated for each component over the concentration range used in this study. CBD was spiked into media containing 1% sodium dodecyl sulfate (SDS). Samples were analyzed using chromatography with UV and mass spectrometry detection. An assessment time of 3 h was chosen as representative of the maximal duration of exposure to gastric fluid. <b><i>Results:</i></b> CBD in SGF with 1% SDS was degraded about 85% after 60 min and more than 98% at 120 min. The degradation followed first-order kinetics at a rate constant of -0.031 min^-1 (<i>R</i>²=0.9933). The major products formed were Δ⁹-THC and Δ⁸-THC with less significant levels of other related cannabinoids. CBD in physiological buffer performed as a control did not convert to THC. Confirmation of THC formation was demonstrated by comparison of mass spectral analysis, mass identification, and retention time of Δ⁹-THC and Δ⁸-THC in the SGF samples against authentic reference standards. <b><i>Conclusions:</i></b> SGF converts CBD into the psychoactive components Δ⁹-THC and Δ⁸-THC. The first-order kinetics observed in this study allowed estimated levels to be calculated and indicated that the acidic environment during normal gastrointestinal transit can expose orally CBD-treated patients to levels of THC and other psychoactive cannabinoids that may exceed the threshold for a physiological response. Delivery methods that decrease the potential for formation of psychoactive cannabinoids should be explored.