Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting

2016627 citationsJournal Article

Authors

Andrew J. Aguirre · Harvard University

Robin M. Meyers

Barbara A. Weir · Dana-Farber Cancer Institute

Francisca Vázquez · Dana-Farber Cancer Institute

Cheng‐Zhong Zhang · Dana-Farber Cancer Institute

Uri Ben‐David

April Cook · Dana-Farber Cancer Institute

Gavin Ha

Abstract

We found that the number of CRISPR/Cas9-induced DNA breaks dictates a gene-independent antiproliferative response in cells. These observations have practical implications for using CRISPR/Cas9 to interrogate cancer gene function and illustrate that cancer cells are highly sensitive to site-specific DNA damage, which may provide a path to novel therapeutic strategies. Cancer Discov; 6(8); 914-29. ©2016 AACR.See related commentary by Sheel and Xue, p. 824See related article by Munoz et al., p. 900This article is highlighted in the In This Issue feature, p. 803.

Topics & Keywords

CRISPR and Genetic Engineering Pluripotent Stem Cells Research Advanced biosensing and bioanalysis techniques

UN Sustainable Development Goals

Good health and well-being

Publication Details

Published in: Cancer Discovery

Volume 6, Issue 8, pp. 914-929

DOI: 10.1158/2159-8290.cd-16-0154

Field-Weighted Citation Impact: 46.22