Genetically Modified Organisms

am er ism spa duplex continuous-flow PCR was also reported for the detection of the P35S and Tnos sequences in GMOs ant D ave br the yi quality oducti PCR system is important, and performing the rapid PCR is difficult in a conventional PCR instrument using a heating/cooling block of large capacity. Therefore, some research groups have made an at-tempt to develop microfluidics-based PCR biomicrofluidic devices [16,17]. Currently, there are two formats of microfluidic PCR de-vices [18–20]: microchamber stationary PCR and continuous-flow PCR. The former is the miniaturization of conventional PCR in nat-ure where the PCR mixture is stationary in the chamber and the temperature is cycled repeatedly [21–23]. However, the chamber stationary PCR microfluidics lacks the flexibility to change the