Characterization of Two Strains of Tribeč Virus Isolated in Ukraine

20148 citationsJournal Article

Authors

Vladimir G. Dedkov · Central Research Institute of Epidemiology

Dmitriy A. Dubina · Ukrainian Research Institute of Transport Medicine

Oksana O. Yurchenko · Ukrainian Research Institute of Transport Medicine

Marina V. Bekova · Central Research Institute of Epidemiology

Anna V. Valdokhina · Central Research Institute of Epidemiology

German A. Shipulin · Central Research Institute of Epidemiology

Abstract

During arbovirus surveillance in Ixodes ricinus ticks in South Ukraine, two strains of Tribeč virus were isolated using an in vivo method and characterized using the complement fixation text (CFT), hemagglutination assay (HA), electron microscopy, and molecular methods. Both strains replicated well in the BHK-21, PEC, and Vero cell lines and demonstrated 95% nucleotide identity in their VP3 sequences compared with the reference VP3 sequence for Tribeč virus. These two strains of Tribeč virus were named Tr35 and Tr19. Also, segment reassortment involving Tr35, Tr19, TRBV, and LIPV strains was shown.

Topics & Keywords

Viral Infections and Vectors Vector-Borne Animal Diseases Vector-borne infectious diseases

UN Sustainable Development Goals

Life in Land

Publication Details

Published in: Vector-Borne and Zoonotic Diseases

Volume 14, Issue 11, pp. 808-816

DOI: 10.1089/vbz.2014.1683

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