Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm

2016528 citationsJournal Articlegold Open Access

Authors

Li‐Fang Chu · Morgridge Institute for Research

Ning Leng · Sunesis (United States)

Jue Zhang · Morgridge Institute for Research

Zhonggang Hou · Harvard Stem Cell Institute

Daniel Mamott · Morgridge Institute for Research

David Vereide · Morgridge Institute for Research

Jeea Choi ·

Abstract

We report the analysis of 1776 cells by scRNA-seq covering distinct human embryonic stem cell-derived progenitor states. By reconstructing a differentiation trajectory at single-cell resolution, novel regulators of the mesendoderm transition to DE are elucidated and validated. Our strategy of combining single-cell analysis and genetic approaches can be applied to uncover novel regulators governing cell fate decisions in a variety of systems.

Topics & Keywords

Pluripotent Stem Cells Research Single-cell and spatial transcriptomics CRISPR and Genetic Engineering

Publication Details

Published in: Genome biology

Volume 17, Issue 1, pp. 173-173

DOI: 10.1186/s13059-016-1033-x

Field-Weighted Citation Impact: 19.88

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Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm

Authors

Abstract

Topics & Keywords

Publication Details