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Aquaporins are membrane integral proteins responsible for the transmembrane transport of water and other small neutral molecules. Despite their well-acknowledged importance in water transport, their significance in gas transport processes remains unclear. Growing evidence points to the involvement of plant aquaporins in CO<sub>2</sub> delivery for photosynthesis. The role of these channel proteins in the transport of O<sub>2</sub> and other gases may also be more important than previously envisioned. In this study, we examined O<sub>2</sub> permeability of various human, plant, and fungal aquaporins by co-expressing heterologous aquaporin and myoglobin in yeast. Two of the most promising O<sub>2</sub>-transporters (Homo sapiens AQP1 and Nicotiana tabacum PIP1;3) were confirmed to facilitate O<sub>2</sub> transport in the spectrophotometric assay using yeast protoplasts. The over-expression of NtPIP1;3 in yeasts significantly increased their O<sub>2</sub> uptake rates in suspension culture. In N. tabacum roots subjected to hypoxic hydroponic conditions, the transcript levels of the O<sub>2</sub>-transporting aquaporin NtPIP1;3 significantly increased after the seven-day hypoxia treatment, which was accompanied by the increase of ATP levels in the apical root segments. Our results suggest that the functional significance of aquaporin-mediated O<sub>2</sub> transport and the possibility of controlling the rate of transmembrane O<sub>2</sub> transport should be further explored.