3D-Printed Bioactive Ca₃SiO₅ Bone Cement Scaffolds with Nano Surface Structure for Bone Regeneration

Silicate bioactive materials have been widely studied for bone regeneration because of their eminent physicochemical properties and outstanding osteogenic bioactivity, and different methods have been developed to prepare porous silicate bioactive ceramics scaffolds for bone-tissue engineering applications. Among all of these methods, the 3D-printing technique is obviously the most efficient way to control the porous structure. However, 3D-printed bioceramic porous scaffolds need high-temperature sintering, which will cause volume shrinkage and reduce the controllability of the pore structure accuracy. Unlike silicate bioceramic, bioactive silicate cements such as tricalcium silicate (Ca₃SiO₅ and C₃S) can be self-set in water to obtain high mechanical strength under mild conditions. Another advantage of using C₃S to prepare 3D scaffolds is the possibility of simultaneous drug loading. Herein, we, for the first time, demonstrated successful preparation of uniform 3D-printed C₃S bone cement scaffolds with controllable 3D structure at room temperature. The scaffolds were loaded with two model drugs and showed a loading location controllable drug-release profile. In addition, we developed a surface modification process to create controllable nanotopography on the surface of pore wall of the scaffolds, which showed activity to enhance rat bone-marrow stem cells (rBMSCs) attachment, spreading, and ALP activities. The in vivo experiments revealed that the 3D-printed C₃S bone cement scaffolds with nanoneedle-structured surfaces significantly improved bone regeneration, as compared to pure C₃S bone cement scaffolds, suggesting that 3D-printed C₃S bone cement scaffolds with controllable nanotopography surface are bioactive implantable biomaterials for bone repair.