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Breast cancer is one of the main causes of women mortality worldwide. Ultrasonography (USG) is other modalities than mammography that capable to support radiologists in diagnosing breast cancer. However, the diagnosis may come with different interpretation depending on the radiologists experience. Therefore, Computer-Aided Diagnosis (CAD) is developed as a tool for radiologist's second opinion. CAD is built based on digital image processing of ultrasound (US) images which consists of several stages. Lesion segmentation is an important step in CAD system because it contains many important features for classification process related to lesion characteristics. This study provides a performance analysis and comparison of image segmentation for breast USG images. In this paper, several methods are presented such as a comprehensive comparison of adaptive thresholding, fuzzy C-Means (FCM), Fast Global Minimization for Active Contour (FGMAC) and Active Contours Without Edges (ACWE). The performance of these methods are evaluated with evaluation metrics Dice coefficient, Jaccard coefficient, FPR, FNR, Hausdorff distance, PSNR and MSSD parameters. Morphological operation is able to increase the performance of each segmentation methods. Overall, ACWE with morphological operation gives the best performance compare to the other methods with the similarity level of more than 90%.