Textural, Structural and Biological Evaluation of Hydroxyapatite Doped with Zinc at Low Concentrations

The present work was focused on the synthesis and characterization of hydroxyapatite doped with low concentrations of zinc (Zn:HAp) (0.01 < <i>x</i>_Zn < 0.05). The incorporation of low concentrations of Zn²⁺ ions in the hydroxyapatite (HAp) structure was achieved by co-precipitation method. The physico-chemical properties of the samples were characterized by X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), Scanning Electron Microscopy (SEM), zeta-potential, and DLS and N₂-BET measurements. The results obtained by XRD and FTIR studies demonstrated that doping hydroxyapatite with low concentrations of zinc leads to the formation of a hexagonal structure with lattice parameters characteristic to hydroxyapatite. The XRD studies have also shown that the crystallite size and lattice parameters of the unit cell depend on the substitutions of Ca²⁺ with Zn²⁺ in the apatitic structure. Moreover, the FTIR analysis revealed that the water content increases with the increase of zinc concentration. Furthermore, the Energy Dispersive X-ray Analysis (EDAX) and XPS analyses showed that the elements Ca, P, O, and Zn were found in all the Zn:HAp samples suggesting that the synthesized materials were zinc doped hydroxyapatite, Ca_10-<i>x</i>Zn<i>_x</i>(PO₄)₆(OH), with 0.01 ≤ <i>x</i>_Zn ≤ 0.05. Antimicrobial assays on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> bacterial strains and HepG2 cell viability assay were carried out.