KRAS mutation analysis of single circulating tumor cells from patients with metastatic colorectal cancer

201750 citationsJournal Articlegold Open Access

Authors

Yuurin Kondo · Tokyo Women's Medical University

Kazuhiko Hayashi · Tokyo Women's Medical University

Kazuyuki Kawakami · Tokyo Women's Medical University

Yukari Miwa · SRL (Japan)

Hiroshi Hayashi · SRL (Japan)

Masakazu Yamamoto · Tokyo Women's Medical University

Abstract

We successfully isolated single CTCs and detected KRAS mutations in individual cells from clinical samples using a novel application of single-cell isolation system. Using the system, we detected CTC heterozygosity and heterogeneity in KRAS status among CTCs within a patient and between CTCs and tumor tissues.

Topics & Keywords

Cancer Cells and Metastasis Cancer Genomics and Diagnostics 3D Printing in Biomedical Research

UN Sustainable Development Goals

Good health and well-being

Publication Details

Published in: BMC Cancer

Volume 17, Issue 1, pp. 311-311

DOI: 10.1186/s12885-017-3305-6

Field-Weighted Citation Impact: 3.53