Magnesium Sulfate Salt Solutions and Ices Fail to Protect <i>Serratia liquefaciens</i> from the Biocidal Effects of UV Irradiation under Martian Conditions

The growth of Serratia liquefaciens has been demonstrated under martian conditions of 0.7 kPa (7 mbar), 0°C, and CO₂-enriched anoxic atmospheres (Schuerger et al., 2013, Astrobiology 13:115-131), but studies into the survivability of cells under hypersaline conditions that are likely to be encountered on Mars are lacking. Serratia liquefaciens cells were suspended in aqueous MgSO₄ solutions, or frozen brines, and exposed to terrestrial (i.e., 101.3 kPa, 24°C, O₂/N₂-normal atmosphere) or martian (i.e., 0.7 kPa, -25°C, CO₂-anoxic atmosphere) conditions to assess the roles of MgSO₄ and UV irradiation on the survival of S. liquefaciens. Four solutions were tested for their capability to attenuate martian UV irradiation in both liquid and frozen forms: sterile deionized water (SDIW), 10 mM PO₄ buffer, 5% MgSO₄, and 10% MgSO₄. None of the solutions in either liquid or frozen forms provided enhanced protection against martian UV irradiation. Sixty minutes of UV irradiation reduced cell densities from 2.0 × 10⁶ cells/mL to less than 10 cells/mL for both liquid and frozen solutions. In contrast, 3-4 mm of a Mars analog soil were sufficient to attenuate 100% of UV irradiation. Results suggest that terrestrial microorganisms may not survive on Sun-exposed surfaces on Mars, even if the cells are embedded in frozen martian brines composed of MgSO₄. However, if dispersed microorganisms can be covered by only a few millimeters of dust or regolith, long-term survival is probable. Key Words: Hypobaria-Mars-Planetary protection-Brines. Astrobiology 17, 401-412.