Search for a command to run...
Female-factor infertility can be caused by poor oocyte quality and depleted ovarian reserves. Egg precursor cells (EPCs), isolated from the ovarian cortex, have the potential to be used to overcome female infertility. We aimed to define the origins of EPCs by analyzing their gene expression profiles and mtDNA content using a mini-pig model. We characterized FAC-sorted DDX4<sup>+</sup>-derived porcine EPCs by performing RNA-sequencing and determined that they utilize pathways important for cell cycle and proliferation, which supports the existence of adult mitotically active oogonial cells. Expression of the pluripotent markers <i>Sox2</i> and <i>Oct4</i>, and the primitive germ cell markers <i>Blimp1</i> and <i>Stella</i> were not detected. However, <i>Nanog</i> and <i>Ddx4</i> were expressed, as were the primitive germ cell markers <i>Fragilis</i>, <i>c-Kit</i> and <i>Tert</i>. Moreover, porcine EPCs expressed self-renewal and proliferation markers including <i>Myc</i>, <i>Esrrb</i>, <i>Id2</i>, <i>Klf4</i>, <i>Klf5</i>, <i>Stat3</i>, <i>Fgfr1</i>, <i>Fgfr2</i> and <i>Il6st</i>. The presence of <i>Zp1</i>, <i>Zp2</i>, <i>Zp3</i> and <i>Nobox</i> were not detected, indicating that porcine EPCs are not indicative of mature primordial oocytes. We performed mitochondrial DNA Next Generation Sequencing and determined that one mtDNA variant harbored by EPCs was present in oocytes, preimplantation embryos and somatic tissues over three generations in our mini-pig model indicating the potential germline origin of EPCs.