Impact of cellular health conditions on the protein folding state in mammalian cells

201753 citationsJournal Articlehybrid Open Access

Authors

Kohsuke Inomata · Kawaguchi Junior College

Hajime Kamoshida · Tokyo Metropolitan University

Masaomi Ikari · Quantitative Biology Center

Yutaka Ito · Tokyo Metropolitan University

T. Kigawa · Tokyo Institute of Technology

Abstract

By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.

Topics & Keywords

Enzyme Structure and Function Protein Structure and Dynamics Endoplasmic Reticulum Stress and Disease

Publication Details

Published in: Chemical Communications

Volume 53, Issue 81, pp. 11245-11248

DOI: 10.1039/c7cc06004a

Field-Weighted Citation Impact: 2.19

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Impact of cellular health conditions on the protein folding state in mammalian cells

Authors

Abstract

Topics & Keywords

Publication Details