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Water-soluble polysaccharides isolated from the rhizome of <i>Polygonatum sibiricum</i> and fractionated using ion-exchange chromatography were investigated to determine their structure and immunostimulating activity. Crude and fractions (F<sub>1</sub> and F<sub>2</sub>) consisted of carbohydrates (85.1~88.3%) with proteins (4.51~11.9%) and uronic acid (1.79~7.47%), and included different levels of mannose (62.3~76.3%), glucose (15.2~20.3%), galactose (4.35~15.3%), and arabinose (4.00~7.65%). The crude contained two peaks with molecular weights (Mw) of 151×10<sup>3</sup> and 31.8×10<sup>3</sup>, but F<sub>1</sub> and F<sub>2</sub> exhibited one major peak with Mw of 103×10<sup>3</sup> and 628×10<sup>3</sup>, respectively. Little immunostimulatory activity was observed by the crude; however, F<sub>1</sub> and F<sub>2</sub> significantly activated RAW264.7 cells to release nitric oxide and various cytokines, suggesting they were potent immunostimulators. The backbone of the most immunostimulating fraction (F<sub>1</sub>) was (1→4)-manno- and (1→4)-gluco-pyranosyl residues with galactose and glucose attached to <i>O</i>-6 of manno-pyranoside.
Published in: Dialnet (Universidad de la Rioja)
Volume 21, Issue 3, pp. 433-433