LINC00152 down-regulated <i>miR-193a-3p</i> to enhance MCL1 expression and promote gastric cancer cells proliferation

The present work aimed to probe into the effect of long non-coding RNA (lncRNA) LINC00152 on gastric cancer (GC) cells proliferation by regulating <i>miR-193a-3p</i> and its target gene <i>MCL1</i> Transfected si-LINC00152 was used to down-regulate LINC00152, and cells proliferation was measured by the cell counting kit-8 (CCK-8) assay. Cell apoptosis and cell cycle were analyzed by flow cytometry (FCM). Besides, we also detected the potential functional effects of differential expression of LINC00152 <i>in vivo</i> using nude mouse xenograft model. We overexpressed and downexpressed <i>miR-193a-3p</i> to study the <i>in vitro</i> effect of <i>miR-193a-3p</i> on GC cells proliferation and vitality. And <i>MCL1</i> was silenced by shRNA to investigate the effect of <i>MCL1</i> on proliferation of GC cells. In this research, LINC00152 was proven to have a higher expression level in GC tissues than in the adjacent normal tissues. GC cells proliferation was inhibited after LINC00152 was down-regulated. LINC00152 inhibited the expression of <i>miR-193a-3p</i>, which negatively regulated <i>MCL1</i> In addition, GC cells proliferation was inhibited by cell transfection with shRNA-<i>MCL1</i>, and enhanced by transfection with <i>miR-193a-3p</i> mimics. Our study suggested that LINC00152 was overexpressed in GC tissues, and it down-regulated <i>miR-193a-3p</i> to enhance <i>MCL1</i> expression thereby promoting GC cells proliferation.