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The fluidic force microscopy (FluidFM) technology provides a versatile force-sensitive nanopipette that can be operated in physiological environments. In addition, the technology allows for real-time monitoring by optical microscopy and for the study of live single cells under preservation of the cellular and physiological context. This chapter provides a synthesis of the complementary applications, starting with the spatial cell manipulation and controlled fluid delivery, which allow for selective and well-defined cell perturbations. It describes single-cell analysis applications that comprise quantitative measurements of cell mechanical and electrical properties and the sampling and analysis of intracellular soluble molecules. FluidFM combines atomic force microscopy (AFM) with microfluidics. Spatial cell manipulation also allows to isolate selected individual cells for downstream analysis or clonal expansion or to designate specific cell localization in cell-based sensors. The extracellular fluid delivery with microscale resolution can be used to perturb individual cells by altering their microenvironment or by stimulation with cell-permeable compounds.