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You have accessJournal of UrologyKidney Cancer: Basic Research & Pathophysiology III1 Apr 2018MP88-20 DNAJB8 EXPRESSION IS ASSOCIATED WITH RECURRENCE OF RENAL CELL CARCINOMA Satoshi Nishizawa, Yuya Iwahashi, Haruka Miyai, Yuko Ueda, Takahito Wakamiya, Takashi Iguchi, Shimpei Yamashita, Akinori Iba, Yasuo Kohjimoto, and Isao Hara Satoshi NishizawaSatoshi Nishizawa More articles by this author , Yuya IwahashiYuya Iwahashi More articles by this author , Haruka MiyaiHaruka Miyai More articles by this author , Yuko UedaYuko Ueda More articles by this author , Takahito WakamiyaTakahito Wakamiya More articles by this author , Takashi IguchiTakashi Iguchi More articles by this author , Shimpei YamashitaShimpei Yamashita More articles by this author , Akinori IbaAkinori Iba More articles by this author , Yasuo KohjimotoYasuo Kohjimoto More articles by this author , and Isao HaraIsao Hara More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2940AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We previously reported the essential functions of novel cancer stem-like cells (CSCs) antigen, DNAJB8 and its crucial regulator, heat shock factor 1 (HSF1). DNAJB8 is preferentially expressed in renal CSCs in comparison with non-CSCs and plays an important role in maintenance of stemness in CSCs. On the other hand, HSF1 is reported highly expressed in several origins of cancers. In this study, we analyzed the expression levels of these molecules in human renal cell carcinoma (RCC) and evaluated the relationships with clinical outcomes. METHODS We collected RCC samples from 51 patients who underwent radical or partial nephrectomy. Total RNA was extracted from RCC specimens and mRNA expressions of DNAJB8 and HSF1 were measured by real-time PCR. Stratified according to the quantitative estimation of the mRNA, the relationship between clinico-pathological data and their expression levels were analyzed. RESULTS Mean age was 64.3 years. Male and female patients were 28 and 23, respectively. Clinical stage was as follows; Stage I: 33, II: 5, III: 8, IV: 5. Histopatological findings showed 48 of clear cell carcinomas and 3 of type II papillary RCCs. Fourteen showed no expression of DNAJB8, all of whom provided no recurrences of cancer after nephrectomy compared to 9 cases of recurrence with DNAJB8 expressed RCCs during follow up period (P=0.05, Log rank test), though no significant association with recurrence was observed by HSF1 expression. DNAJB8 expression was significantly associated with pathological vascular invasion (P=0.04) and tended to be related to cytological grade (P=0.09). Infiltrative growth pattern (INF) was not associated with DNAJB8 expression (P=0.43). HSF1 expression did not affect pathological features. CONCLUSIONS CSCs are assumed to contribute on recurrence after curative treatment such as radical surgery or radiotherapy. CSCs antigen DNAJB8 was confirmed to be associated with cancer recurrence and pathological features. DNAJB8 might be a good targeting molecule at RCC patients with high risk of recurrence. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e1205 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Satoshi Nishizawa More articles by this author Yuya Iwahashi More articles by this author Haruka Miyai More articles by this author Yuko Ueda More articles by this author Takahito Wakamiya More articles by this author Takashi Iguchi More articles by this author Shimpei Yamashita More articles by this author Akinori Iba More articles by this author Yasuo Kohjimoto More articles by this author Isao Hara More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...