Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

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Authors

Chrysanthia A. Leontiou · Cyprus Institute of Neurology and Genetics

Michael D. Hadjidaniel · Cyprus Institute of Neurology and Genetics

Petros Mina · Cyprus Institute of Neurology and Genetics

Pavlos Antoniou · Medicover Genetics (Cyprus)

Marios Ioannides · Cyprus Institute of Neurology and Genetics

Philippos C. Patsalis · Cyprus Institute of Neurology and Genetics

Abstract

Our findings indicate that the MethylEdge Bisulfite Conversion System (Promega) was shown to have the best performance among the kits. In addition, the methylation level of two of our DMRs, EP6 and EP10, was confirmed. Finally, we showed that bisulfite amplicon sequencing is a suitable approach for methylation analysis of targeted regions.

Topics & Keywords

Epigenetics and DNA Methylation Prenatal Screening and Diagnostics Genetic Syndromes and Imprinting

Publication Details

Published in: PLoS ONE

Volume 10, Issue 8, pp. e0135058-e0135058

DOI: 10.1371/journal.pone.0135058

Field-Weighted Citation Impact: 2.11

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Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

Authors

Abstract

Topics & Keywords

Publication Details