Genetic Screening of Cells with Enhanced Antibody Production

Abstract : Here we report the progress made for grant No. W911NF-05-C-0059 between the entire funding period, 6/1/05 and 8/31/06. We have completed the study proposed: to use Functional Genetics' proprietary technology, Randon Homozygous Knock Out (RHKO) to increase antibody production from a production cell line, in this case CHO cells, as measured by Specific Productivity Rate (SPR). We also identified 2 candidate RHKO genes that cause increased SPR. Finally, we report the validation of one of these two genes.