IN-VITRO STUDY ON THE HEMOLYTIC ACTIVITY OF DIFFERENT EXTRACTS OF INDIAN MEDICINAL PLANT CROTON BONPLANDIANUM WITH PHYTOCHEMICAL ESTIMATION: A NEW ERA IN DRUG DEVELOPMENT

In this study different extracts of the leaves of Croton bonplandianum were screened for the haemolytic activity towards human erythrocytes. The haemolytic activity was performed by modified spectroscopic method at four different concentrations (300, 150, 75, 25 μg/ml). The haemolytic activity of the different extracts of Croton bonplandianum was found in the following order Ethyl acetate extract > Chloroform extract > Benzene extract. However, all the extracts alone and in combination with each other exhibited very low haemolytic activity. E. ganitrus did not exhibit any haemolytic activity at any dilution. Hence, they can be considered as safe to human erythrocytes. Keywords: Hemolytic activity, Croton bonplandianum, Erythrocytes