Chromogenic, Fluorescent, and Redox Sensors for Multichannel Imaging and Detection of Hydrogen Peroxide in Living Cell Systems

Hydrogen peroxide (H₂O₂) is an important reactive oxygen species (ROS). Maintaining the H₂O₂ concentration at a normal level is critical to achieve the normal physiological activities of cells, which otherwise might trigger various diseases. Therefore, it is necessary to develop new and practical multisignaling sensors for both visualization of intracellular H₂O₂ and accurate detection of extracellular H₂O₂. In this paper, a novel multichannel signaling fluorescence-electrochemistry combined probe 1 (FE-H₂O₂) is presented for imaging and detection of H₂O₂ in living cell systems. In our design, the probe FE-H₂O₂ consists of a H₂O₂ reaction site and 4-ferrocenyl(vinyl)pyridine unit which affords chromogenic, fluorescent, and electrochemical signals. These structural motifs yield a combined chromogenic, fluorescent, and redox sensor in a single molecule. Probe FE-H₂O₂ showed a "Turn-On" fluorescence response to H₂O₂, which can be used for monitoring intracellular H₂O₂ in vivo. Furthermore, the electrochemical response of probe FE-H₂O₂ was decreased after the addition of H₂O₂, which can be applied for accurate detection of H₂O₂ released from living cells. When the fluorescence imaging method is combined with electrochemical analysis technology, it is hopeful that the well-designed multimodule probe can serve as a practical tool for understanding the metabolism and homeostasis of H₂O₂ in a complex biological system.