Engineering of CYP76AH15 can improve activity and specificity towards forskolin biosynthesis in yeast

201853 citationsJournal Articlegold Open Access

Authors

Birger Lindberg Møller · University of Copenhagen

Abstract

As demonstrated for CYP76AH15, site-directed mutagenesis of SRS regions of plant CYPs may be an efficient and targeted approach to increase the performance of these enzymes. Although in this work we have managed to achieve higher efficiency and specificity of the first CYP of the pathway, further work is necessary in order to increase the overall production of forskolin in yeast cells.

Topics & Keywords

Plant biochemistry and biosynthesis Microbial Natural Products and Biosynthesis Photosynthetic Processes and Mechanisms

UN Sustainable Development Goals

Responsible consumption and production

Publication Details

Published in: Microbial Cell Factories

Volume 17, Issue 1, pp. 181-181

DOI: 10.1186/s12934-018-1027-3

Field-Weighted Citation Impact: 1.67