Laccase pretreatment of wheat straw: effects of the physicochemical characteristics and the kinetics of enzymatic hydrolysis

The adsorption ability of the enzyme to lignin was reduced after LMS pretreatment. Physicochemical analyses showed that the chemical groups of lignin and lignocellulose were changed, with the crystallinity of the lignocellulose increasing after LMS treatment. A semi-empirical kinetic model was proposed to estimate the reducing sugar yield, which showed high accuracy for predicting the hydrolysis performance of laccase-treated wheat straw.