Initial Results from the CryoARM300 and DE-64 Counting

We have demonstrated the use of the CryoARM300 with cold-FEG and the DE-64 Camera operating in electron counting mode by carrying out automated single-particle cryo-EM data acquisition of apoferritin. Data was acquired with JADAS automation software Reconstruction of this initial data set with Relion [6] yielded a 3.6 resolution structure (Fig. With this workflow now established, we can now test the limits of data quality and throughput on the CryoARM300 and .