Assembly and Analysis of the Genome Sequence of the Yeast Brettanomyces naardenensis CBS 7540

<i>Brettanomyces naardenensis</i> is a spoilage yeast with potential for biotechnological applications for production of innovative beverages with low alcohol content and high attenuation degree. Here, we present the first annotated genome of <i>B. naardenensis</i> CBS 7540. The genome of <i>B. naardenensis</i> CBS 7540 was assembled into 76 contigs, totaling 11,283,072 nucleotides. In total, 5168 protein-coding sequences were annotated. The study provides functional genome annotation, phylogenetic analysis, and discusses genetic determinants behind notable stress tolerance and biotechnological potential of <i>B. naardenensis</i>.