Green synthesis of zinc oxide nanostructures and investigation of their photocatalytic and bactericidal applications

We report a facile one-pot green synthesis of zinc oxide (ZnO) nanostructures using aqueous leaf extract of <i>Dolichos Lablab</i> L. as the reducing and capping agent. The optical properties, structure and morphology of the as-synthesized ZnO nanostructures have been characterized by UV-Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) supported with energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). TEM analysis revealed that the as-synthesized ZnO nanostructures have an average particle diameter of 29 nm. XRD patterns confirmed the formation of phase-pure ZnO nanostructures with a hexagonal wurtzite structure. The synthesized ZnO nanostructures were used as a catalyst in the photodegradation of methylene blue (MB), rhodamine B (RhB) and orange II (OII) under visible and near-UV irradiation. The results showed the highest efficiency of photodegradation of ZnO nanostructures for MB (80%), RhB (95%) and OII (66%) at pH values of 11, 9 and 5, respectively, in a 210 min time interval. In addition, the antimicrobial activity of the ZnO nanostructures using the agar well diffusion method against <i>Bacillus pumilus</i> and <i>Sphingomonas paucimobilis</i> showed the highest zones of inhibition of 18 mm and 20 mm, respectively. Hence, ZnO nanostructures have the potential to be used as a photocatalyst and bactericidal component.