Distinct diagnostic and prognostic values of γ‑aminobutyric acid type A receptor family genes in patients with colon adenocarcinoma

In the present study, the significance of <i>GABA_A</i> genes in colon adenocarcinoma (COAD) were investigated from the view of diagnosis and prognosis. All data were achieved from The Cancer Genome Atlas. Overall survival was analyzed by the Kaplan-Meier analyses and Cox regression model and the hazard ratios and 95% confidence interval were calculated for computation. The Database for Annotation, Visualization and Integrated Discovery, and the Biological Networks Gene Ontology (BiNGO) softwares were applied to assess the biological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for pathway analysis to predict the biological function of <i>GABA_A</i> genes. The associated Gene Ontology and KEGG pathways were conducted by Gene Set Enrichment Analysis (GSEA). From receiver operating characteristics curves analysis, it was found that the expression of <i>GABR</i>, γ-aminobutyric acid type A receptor <i>GABRA2, GABRA3, GABRB2, GABRB3, GABRG2, GABRG3, GABRD, GABRE</i> were correlated with COAD occurrence [P<0.0001, area under the curve (AUC)>0.7]. The low expression of the <i>GABRB1, GABRD, GABRP</i> and <i>GABRQ</i> in genes after tumor staging adjustment were positively correlated with the overall survival rate [P=0.049, hazard ratio (HR)=1.517, 95% confidence interval (CI)=1.001-2.297; P=0.006, HR=1.807, 95% CI=1.180-2.765; P=0.005, HR=1.833, 95% CI=1.196-2.810; P=0.034, HR=1.578, 95% CI=1.036-2.405). GSEA showed enrichment of cell matrix adhesion, integrin binding, angiogenesis, endothelial growth factor and endothelial migration regulation in patients with COAD with <i>GABRD</i> overexpression. <i>GABRB1, GABRD, GABRP and GABRQ</i> were associated with the prognostic factors of COAD. The expression levels of <i>GABRA2, GABRA3, GABRB2, GABRB3, GABRG2, GABRD</i> and <i>GABRE</i> may allow differentiation between tumor tissues and adjacent normal tissues.