Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

2020814 citationsReviewgold Open Access

Authors

Rupsa Datta · Morgridge Institute for Research

Tiffany M. Heaster · University of Wisconsin–Madison

Joe T. Sharick · Morgridge Institute for Research

Amani A. Gillette · Morgridge Institute for Research

Melissa C. Skala · Morgridge Institute for Research

Abstract

FLIM is advantageous for probing molecular environments of fluorophores to inform on fluorophore behavior that cannot be elucidated with intensity measurements alone. Development of FLIM technologies, analysis, and applications will further advance biological research and clinical assessments.

Topics & Keywords

Advanced Fluorescence Microscopy Techniques Photoacoustic and Ultrasonic Imaging Advanced Electron Microscopy Techniques and Applications

UN Sustainable Development Goals

Affordable and clean energy

Publication Details

Published in: Journal of Biomedical Optics

Volume 25, Issue 07, pp. 1-1

DOI: 10.1117/1.jbo.25.7.071203

Field-Weighted Citation Impact: 83.03