Assessment of fecal DNA extraction protocols for metagenomic studies

202089 citationsJournal Articlegold Open Access

Authors

Fangming Yang · BGI Group (China)

Jihua Sun · University of Copenhagen

Huainian Luo · BGI Europe (Denmark)

Huahui Ren · BGI Group (China)

Hongcheng Zhou · China National GeneBank

Yuxiang Lin · BGI Group (China)

Mo Han · BGI Group (China)

Abstract

We emphasize the importance of bead size selection for bacterial and fungal DNA extraction. More importantly, the performance of the novel protocol MP matched that of the recommended standardized protocol Q but consumed less time, was more cost-effective, and is recommended for further large-scale human gut metagenomic studies.

Topics & Keywords

Gut microbiota and health Colorectal Cancer Screening and Detection

UN Sustainable Development Goals

Life in Land

Publication Details

Published in: GigaScience

Volume 9, Issue 7

DOI: 10.1093/gigascience/giaa071

Field-Weighted Citation Impact: 3.26