FLOW-CHAMBER MICROARRAY DETECTION OF MALARIAL ANTIGENS

Detecting and quantifying circulating proteins produced by malarial parasites provides valuable information to guide diagnosis and treatment. We report on the detection and quantification of the malarial antigens HRP-II and aldolase with single-use microfluidic cartridges incorporating protein microarrays. The microarray-based cartridges are suitable for use with handheld bioassay instruments. Reagent and sample transport in the cartridges is controlled by integrated silicon electroosmotic micropumps. Applications of the flow-chamber microarrays for experimentally determining binding parameters for pairs of biological molecules are explored.