Systematic Analysis of Combined Antioxidant and Membrane-Stabilizing Properties of Several Lamiaceae Family Kazakhstani Plants for Potential Production of Tea Beverages

One of the most important compounds that exhibit a wide range of biological activities with especially strong antioxidant action are plant polyphenols. In the course of the experiment, the dose-dependent effects of polyphenols-rich extracts isolated from the <i>Lamiaceae</i> family Kazakhstani plants were studied on the processes of lipid peroxidation and on the degree of erythrocytes hemolysis. The activity of aqueous-ethanolic extracts from dried parts of plants, such as <i>Origanum vulgare</i>, <i>Ziziphora bungeana</i>, <i>Dracocephalum integrifolium</i>, <i>Mentha piperita</i>, <i>Leonurus turkestanicus</i>, <i>Thymus serpyllum</i>, and <i>Salvia officinalis</i>, was studied in a Wistar rat model. Lipid peroxidation (LPO) in liver microsomes was assessed by measuring malondialdehyde content in the form of thiobarbituric acid-reacting substances (TBARS). Estimation of osmotic resistance of isolated erythrocytes was evaluated based on hemoglobin absorbance. The amount of total phenolics in the extracts was measured using the Folin-Ciocalteu reagent method. Based on the results, <i>Thymus serpyllum</i> extract exhibited a significantly higher antioxidant activity (IC50 = 3.3 ± 0.7) compared to other plant extracts. Accordingly, among the extracts studied, those from <i>Salvia officinalis</i>, <i>Thymus serpyllum</i>, and <i>Origanum vulgare</i> show the most pronounced membrane-stabilizing activity. Antioxidant and antihemolytic properties of green tea and <i>Origanum vulgare</i> extract mixtures were similar to that of each individual plant extract. Similar results were obtained when the green tea extract was mixed with <i>Mentha piperita</i>, <i>Ziziphora bungeana</i>, and <i>Dracocephalum integrifolium</i> extracts, indicating no discernible synergistic interaction.