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With the aim of analysis the influence of temperature-humidity index (THI) on daily performance of dairy cows in Croatian Mediterranean region, 217,509 test-day records collected from January 2005 until April 2010 were extracted from HPA (Croatian Agricultural Agency) database. Milk recording in Croatia occurs according to the alternative milk recording method every four weeks, therefore, daily production of milk yields and components was projected using projection parameters estimated in authors' earlier research (Gantner, 2008). Logical control of data was performed according to ICAR standards (2003). The yields recorded after the 500th lactation day was deleted from the dataset. According to the parity, cows were divided into three classes (heifers, cows in second lactation and cows in third and higher lactations) while, regarding the calving date, cows were divided in two calving season subgroups. According to the test date, four measuring season subgroups were created. Based on temperature and relative humidity recorded in stable at each milking control the daily temperature-humidity index (THI) values were calculated using the equation by Kibler (1964). For estimation of the influence of exceeded THI (above critical value -72) on daily production of dairy cows statistical model that take into account effects of lactation stage, breed, calving season, measuring season as well as effect of THI group (T1 - THI under critical value; T2 - THI above critical value). The significance of differences between the means of the daily milk, fat, protein and lactose yield, as well as between the means of the daily fat, protein, and lactose content within the THI classes in regard to parity classes was tested with Scheffe test. For the statistical analysis and the figures drawing the SAS/STAT package was used (SAS Institute Inc., 2000). The exceeded THI significantly decrease daily milk, fat, protein and lactose yield in heifers and cows in third and higher lactations. In second lactation determined decrease was not significant. Highly significant decrease of daily fat and protein content was determined in all cows, regardless the parity. The daily lactose content was not significantly influenced by enhanced value of THI. Management strategies are needed to minimize heat stress and to attain optimal cows' production in climatic condition of Croatian Mediterranean region.