The Response of Muscle Protein Anabolism to Combined Hyperaminoacidemia and Glucose-Induced Hyperinsulinemia Is Impaired in the Elderly

Muscle mass declines with aging.Amino acids alone stimulate muscle protein synthesis in the elderly.However, mixed nutritional supplementation failed to improve muscle mass.We hypothesized that the failure of nutritional supplements is due to altered responsiveness of muscle protein anabolism to increased amino acid availability associated with endogenous hyperinsulinemia.We measured muscle protein synthesis and breakdown, and amino acid transport in healthy young (30 ± 3 yr) and elderly (72 ± 1 yr) volunteers in the basal postabsorptive state and during the administration of an amino acid-glucose mixture, using L-[ring-2 H 5 ]phenylalanine infusion, femoral artery and vein catheterization, and muscle biopsies.Basal muscle amino acid turnover was similar in young and elderly subjects.The mixture increased phenylalanine leg delivery and transport into the muscle in both groups.Phenylalanine net balance increased in both groups (young, -27 ± 8 to 64 ± 17; elderly, -16 ± 4 to 29 ± 7 nmol/(min•100 mL); P < 0.0001, basal vs. mixture), but the increase was significantly blunted in the elderly (P = 0.030 vs. young).Muscle protein synthesis increased in the young, but remained unchanged in the elderly [young, 61 ± 17 to 133 ± 30 (P = 0.005); elderly, 62 ± 9 to 70 ± 14 nmol/(min•100 mL) (P = NS)].In both groups, protein breakdown decreased (P = 0.012) and leg glucose uptake increased (P = 0.0258) with the mixture.We conclude that the response of muscle protein anabolism to hyperaminoacidemia with endogenous hyperinsulinemia is impaired in healthy elderly due to the unresponsiveness of protein synthesis.Muscle mass and function progressively decline with aging (1, 2).This process has been termed sarcopenia and is associated with risk of falls and functional dependence (3, 4).Both undernutrition and disuse have been identified as potentially preventable contributing factors (5,6).Nutritional intervention is an appealing method of prevention and treatment of sarcopenia of the elderly due to its wide applicability and safety.However, attempts to