Assay establishment and validation of a high-throughput organoid-based drug screening platform

202246 citationsJournal Articlegold Open Access

Authors

Xiaomeng Li · Shanghai Medical College of Fudan University

Guoxiang Fu

Long Zhang · Shanghai Medical College of Fudan University

Ruo‐Yu Guan · Shanghai Medical College of Fudan University

Peiyuan Tang · Shanghai Medical College of Fudan University

Jialing Zhang · Shanghai Medical College of Fudan University

Xinxin Rao · Fudan University Shanghai Cancer Center

Abstract

In summary, combining the Z-stack imaging technique and fluorescent labeling methods, we established an assay for the imaging and analysis of three-dimensional organoids. Our data demonstrated the feasibility of using organoid-based platforms for high-throughput drug screening assays.

Topics & Keywords

Cancer Cells and Metastasis 3D Printing in Biomedical Research Cell Image Analysis Techniques

UN Sustainable Development Goals

Good health and well-being

Publication Details

Published in: Stem Cell Research & Therapy

Volume 13, Issue 1, pp. 219-219

DOI: 10.1186/s13287-022-02902-3

Field-Weighted Citation Impact: 4.40