Long-term effects of forest fires on fungal community and soil properties along a hemiboreal Scots pine forest fire chronosequence

We studied long-term effects of forest fires on the dynamics of soil fungal community along a post-fire chronosequence in hemiboreal Scots pine stands in north-western Estonia. Effects of fire on soil and fungi were studied on six sites that differed in time since fire (10, 21, 36, 67, 78 and 181 years ago), without further management interventions. Soil fungal communities along the chronosequence were dominated by soil saprotrophs and ectomycorrhizal (EcM) fungi. Across the chronosequence, the most dominant phylum was Ascomycota. The most abundant OTUs were identified as Umbelopsis sp., Hyaloscyphaceae sp. and Pezoloma ericae with relative abundances of 9.5, 8.9 and 6.8 %, respectively. Fungal species richness was similar among sample areas except in the area where fire occurred 36 years ago, where it was significantly lower. There were considerable differences in EcM fungal species composition along the chronosequence. The most recently burned site had Piloderma sphaerosporum, Pseudotomentella sp. and Clavulinaceae sp. as most abundant EcM OTUs while in three oldest burned areas Clavulinaceae sp. and Cortinarius sp. were abundant. Soil C and N stocks were lower in the most recently burned area but differences with other areas were not statistically significant. Soil pH had a significant effect on fungal species composition. Older areas had substantially lower pH compared to more recently burned areas.